Difference Between Cyclic and Non-Cyclic Photophosphorylation

Biology · Photosynthesis in Higher Plants · NEET

Cyclic photophosphorylation uses only Photosystem I (PS I), the electron goes back to PS I, and it makes only ATP (no NADPH, no oxygen). Non-cyclic photophosphorylation uses both PS II and PS I in a series (the Z-scheme), and it makes ATP, NADPH, and oxygen. Memory hook: "Cyclic = Circle back, only ATP; Non-cyclic = New products (NADPH + O2) too."

At a glance

Photosystems usedOnly PS I (P700)Both PS II (P680) and PS I (P700)
Electron pathElectron cycles back to PS IOpen path, electron ends on NADP+
ProductsOnly ATPATP + NADPH + H+
Water splitting / O2No photolysis, no oxygenWater split, oxygen released
Likely siteStroma lamellae (no PS II, no NADP reductase)Grana lamellae (have both PS I and PS II)
When it occursOnly PS I active; light beyond 680 nm; extra ATP neededNormal light, both photosystems active
CYCLIC (only PS I)NON-CYCLIC (PS II + PS I)PS IP700e- cycles backonly ATPno NADPHno O2PS IIP680PS IP700ETCNADP+to NADPHH2O splitO2 out
Cyclic (left): electron leaves PS I and returns to PS I, making only ATP. Non-cyclic (right): electron flows PS II to PS I through the Z-scheme, making ATP, NADPH, and releasing O2 from water splitting.

Your doubts, answered

Does cyclic photophosphorylation produce NADPH?

No. In cyclic photophosphorylation only PS I works and the excited electron is cycled back to PS I through the electron transport chain instead of going to NADP+. So the product is only ATP. NADPH + H+ is made only in non-cyclic photophosphorylation, where the electron finally reaches NADP+ and reduces it. This one point (only ATP vs ATP + NADPH) is the most common NEET question on this topic.

Why does cyclic photophosphorylation not release oxygen?

Oxygen comes from the splitting of water (photolysis), and water splitting is linked to PS II. Cyclic photophosphorylation does not use PS II, so water is not split and no oxygen is released. Non-cyclic photophosphorylation uses PS II, splits water, and therefore releases oxygen. Remember: no PS II means no water splitting means no O2.

Which photosystem is used in cyclic photophosphorylation?

Only PS I (with reaction centre P700) is used in cyclic photophosphorylation. Non-cyclic photophosphorylation uses both PS II (P680) and PS I (P700) working in a series. A very common NEET trap is the statement 'cyclic photophosphorylation involves both PS I and PS II' - this is wrong.

Where in the chloroplast does cyclic photophosphorylation occur?

According to NCERT, a likely site is the stroma lamellae (the stroma thylakoid membranes). The stroma lamellae have PS I only; they lack PS II and lack the NADP reductase enzyme. The grana lamellae have both PS I and PS II, so non-cyclic photophosphorylation happens on the grana thylakoids.

When does cyclic photophosphorylation take place?

Cyclic photophosphorylation occurs when only PS I is functional. NCERT also states it happens when only light of wavelength beyond 680 nm is available (because PS II needs shorter wavelengths to work). It also runs to supply the extra ATP that the Calvin cycle needs, since the cycle uses more ATP than NADPH per CO2 fixed.

⚠️ The NEET trap
Cyclic photophosphorylation involves both PS I and PS II.
Cyclic photophosphorylation involves only PS I. Both photosystems are used only in non-cyclic photophosphorylation.
🧠 NEET repeats this exact line (2019, 2021, ReNEET 2026) as the INCORRECT option. If a statement says cyclic uses both PS I and PS II, it is the wrong statement.

Real NEET questions

2021

Which of the following statements is incorrect?

A · Grana lamellae have both PS I and PS II
B · Cyclic photophosphorylation involves both PS I and PS II.
C · Both ATP and NADPH + H+ are synthesized during non-cyclic photophosphorylation.
D · Stroma lamellae have PS I only and lack NADP reductase
Solution: Cyclic photophosphorylation uses only PS I - the electron is circulated within PS I and recycled back, producing only ATP. It does NOT use PS II. Only non-cyclic photophosphorylation uses both PS II and PS I. So the statement that cyclic involves both photosystems is the incorrect one. (Repeated across NEET 2019, 2021 and ReNEET 2026.)
2023

Which out of the following statements is incorrect?

A · Both ATP and NADPH + H+ are synthesised during non-cyclic photophosphorylation.
B · Stroma lamellae lack PS II and NADP reductase
C · Grana lamellae have both PS I and PS II
D · Cyclic photophosphorylation involves both PS I and PS II
Solution: Cyclic photophosphorylation is driven by PS I alone (P700) in the stroma lamellae, which lack PS II and NADP reductase, so it makes only ATP. The claim that cyclic photophosphorylation involves both PS I and PS II is therefore incorrect. All other statements are correct.

Solved Photosynthesis in Higher Plants NEET PYQs

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Frequently asked

What is the main difference between cyclic and non-cyclic photophosphorylation?

Cyclic uses only PS I and makes only ATP. Non-cyclic uses PS II and PS I together (Z-scheme) and makes ATP, NADPH and oxygen.

Does non-cyclic photophosphorylation produce oxygen?

Yes. Non-cyclic uses PS II, which splits water (photolysis). This water splitting releases oxygen. Cyclic photophosphorylation does not split water, so it releases no oxygen.

Why does the plant need cyclic photophosphorylation at all?

The Calvin cycle needs more ATP than NADPH (3 ATP and 2 NADPH per CO2). Cyclic photophosphorylation makes the extra ATP needed to balance this, since it produces ATP without producing NADPH.

Which is the final electron acceptor in each pathway?

In non-cyclic photophosphorylation the final electron acceptor is NADP+ (forming NADPH). In cyclic photophosphorylation there is no final external acceptor - the electron returns to PS I, so it keeps cycling.

Is oxygen released in cyclic photophosphorylation? A common one-mark question.

No. Cyclic photophosphorylation does not use PS II and does not split water, so no oxygen is released. This is a frequent NEET assertion-reason and single-statement trap.