Difference Between Photosystem I and Photosystem II

Biology · Photosynthesis in Higher Plants · NEET

Photosystem I (PS I) has its reaction centre chlorophyll a absorbing best at 700 nm, so it is called P700. Photosystem II (PS II) has its reaction centre at 680 nm, called P680. The key difference: PS II splits water and releases oxygen, while PS I finally hands electrons to NADP+ to make NADPH. Memory hook: numbers are backwards. PS II works FIRST in the Z-scheme (it starts electron flow and makes O2), PS I works second. They were numbered in the order they were discovered, not the order they work.

At a glance

Reaction centreP680 (chlorophyll a)P700 (chlorophyll a)
Absorption peak680 nm700 nm
Splits water / photolysisYes (water-splitting complex here)No
Releases oxygenYesNo
Order of action in Z-schemeFirstSecond
Final electron outputPasses electrons on to carriersReduces NADP+ to NADPH
Cyclic photophosphorylationNot involvedInvolved (only PS I)
PS II vs PS I in the Z-scheme (light reaction)Photosystem IIReaction centre: P680Splits waterReleases O2Photosystem IReaction centre: P700Passes e- to NADP+Makes NADPHelectrons flow (via carriers)acts FIRSTacts SECONDNamed by order of discovery, not order of working
In non-cyclic flow, electrons start at Photosystem II (P680, splits water, gives O2), pass through carriers, and reach Photosystem I (P700), which makes NADPH. PS II acts first even though PS I has the smaller number.

Your doubts, answered

Why does Photosystem II work before Photosystem I if it has a bigger number?

The photosystems were named in the order scientists discovered them, not the order they work in. In the non-cyclic pathway (Z-scheme), electrons start at PS II, travel through carriers, and end at PS I. So PS II acts first, PS I acts second. NCERT states this directly: they are named in the sequence of their discovery, and not in the sequence in which they function.

Which photosystem splits water and gives out oxygen?

Only Photosystem II (PS II) splits water. The oxygen-evolving (water-splitting) complex is attached to PS II. When water splits, it gives electrons (to refill P680), protons (H+), and oxygen. PS I does NOT split water and does NOT release oxygen. A very common NEET trap is to link the water-splitting complex with PS I, which is wrong.

What is the difference between P700 and P680?

P700 is the reaction centre chlorophyll a of Photosystem I; it absorbs light best at 700 nm. P680 is the reaction centre chlorophyll a of Photosystem II; it absorbs best at 680 nm. Both are single chlorophyll a molecules, but they sit in different photosystems and absorb at different wavelengths. The 'P' stands for pigment and the number is the absorption peak in nanometres.

Which photosystem finally makes NADPH?

Photosystem I is the one whose electrons finally reduce NADP+ to NADPH. Electrons excited from P700 pass to ferredoxin and then to NADP+, forming NADPH on the stroma side. PS II does not make NADPH directly; it starts the electron flow and makes oxygen. So: PS II = water splitting and O2, PS I = NADPH.

Do both photosystems take part in cyclic photophosphorylation?

No. Cyclic photophosphorylation uses only Photosystem I (P700). Electrons leave P700, go around a loop, and come back to P700, making only ATP (no NADPH, no O2). Non-cyclic photophosphorylation uses BOTH PS I and PS II and makes ATP, NADPH and oxygen. This 'which system is used' point is a frequent NEET question.

⚠️ The NEET trap
The water-splitting (oxygen-evolving) complex is part of Photosystem I.
The water-splitting / oxygen-evolving complex is part of Photosystem II (PS II). PS I does not split water and does not release oxygen.
🧠 Water splitting = PS II. Remember: PS II acts first, so it must first grab electrons from water. NEET 2026 marked the statement 'water splitting complex is associated with PS I' as INCORRECT.

Real NEET questions

2023

The reaction centre in PS II has an absorption maxima at

A · 680 nm
B · 700 nm
C · 660 nm
D · 780 nm
Solution: Each photosystem has a single reaction-centre chlorophyll a. In PS II this absorbs maximally at 680 nm and is called P680. (In PS I it is 700 nm, called P700.) So the PS II reaction centre absorbs at 680 nm.
2026

Which pigment has an absorption peak at 700 nm in the photosynthetic reaction centre PS I (P700)?

A · Chlorophyll b
B · Chlorophyll a
C · Xanthophylls
D · Carotenoids
Solution: In Photosystem I the reaction-centre pigment is a single chlorophyll a molecule that absorbs best at 700 nm, so it is called P700. In PS II the reaction-centre chlorophyll a absorbs at 680 nm (P680). The reaction centre is always chlorophyll a, not chlorophyll b or accessory pigments.
2026

Find the incorrect statement(s) about photosynthesis: A. The water splitting complex is associated with PS I. B. C4 plants use the C3 pathway of CO2 fixation as the main biosynthetic pathway. C. In C4 plants, photorespiration does not occur. D. C3 plants exhibit 'Kranz' anatomy. E. ATP synthesis in chloroplast occurs through chemiosmosis.

A · B and C only
B · B only
C · B and E only
D · A and D only
Solution: Statement A is incorrect: the water-splitting (oxygen-evolving) complex belongs to PS II, not PS I. Statement D is incorrect: Kranz anatomy is shown by C4 plants, not C3 plants. B, C and E are correct. So the incorrect statements are A and D.

Solved Photosynthesis in Higher Plants NEET PYQs

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Frequently asked

What is the main difference between PS I and PS II?

PS II splits water and releases oxygen and has reaction centre P680 (680 nm). PS I has reaction centre P700 (700 nm) and finally passes electrons to NADP+ to make NADPH. PS II acts first in the Z-scheme, PS I second.

Why is Photosystem I numbered I if it works second?

Because it was discovered first. NCERT clearly says the photosystems are named in the order of their discovery, not the order in which they function during the light reaction.

Which photosystem is involved in cyclic photophosphorylation?

Only Photosystem I (P700). It makes only ATP, no NADPH and no oxygen. Non-cyclic photophosphorylation uses both PS I and PS II.

Is the reaction centre chlorophyll a or chlorophyll b?

The reaction centre in both photosystems is a single molecule of chlorophyll a. The other pigments (chlorophyll b, carotenoids, xanthophylls) act as accessory light-harvesting pigments in the antenna.

Which photosystem produces oxygen?

Photosystem II produces oxygen through the splitting of water (photolysis). PS I never releases oxygen.