Balanced Overall Equation of Photosynthesis

Biology · Photosynthesis in Higher Plants · NEET

The correct balanced equation of photosynthesis is 6CO2 + 12H2O → C6H12O6 + 6H2O + 6O2 (in light, with chlorophyll). Memory hook: think "6-12 in, glucose + 6-6 out" — 6 CO2 and 12 water go in, and one glucose plus 6 water and 6 oxygen come out. The 6 oxygen molecules all come from water, not from CO2.
Correct Balanced Equation of Photosynthesis6 CO212 H2OReactantslightchlorophyllC6H12O6(glucose)6 H2O6 O2O2 comesfrom water12 water split to release 6 O2; net water used = 12 - 6 = 6
The correct balanced equation: 6 CO2 and 12 H2O react in light with chlorophyll to give glucose, 6 water, and 6 O2. All 6 oxygen molecules come from the splitting of water.

Your doubts, answered

Why is 6H2O written on the product side too?

Because 12 water molecules are used as reactants but only the net change matters. NCERT writes the full balanced form: 6CO2 + 12H2O → C6H12O6 + 6H2O + 6O2. Six water molecules are re-formed during the reaction, so 6 appear again as products. The net water consumed is 12 minus 6 = 6, but the equation must show 12 on the left because 12 waters are actually split.

Why are 12 water molecules used and not 6?

Because the O2 released comes only from water, not from CO2. To release 6 molecules of O2 you must split 12 molecules of water (each O2 needs 2 water molecules). This was proved using radioisotope (heavy oxygen) tracing. If only 6 water were used, the oxygen count would not balance.

Does the oxygen released come from CO2 or water?

From water. Cornelius van Niel showed photosynthesis is a light reaction where hydrogen from a compound reduces CO2. In green plants that hydrogen donor is H2O, which is oxidised to O2. So the O2 evolved comes from the splitting of water (photolysis), not from carbon dioxide. This is why 12 H2O appear in the correct equation.

Is 6CO2 + 6H2O → C6H12O6 + 6O2 wrong then?

It is the older simplified (empirical) form and is not the fully correct one for NEET. NCERT calls the empirical equation 6CO2 + 6H2O → C6H12O6 + 6O2, but after van Niel's work the correct equation became 6CO2 + 12H2O → C6H12O6 + 6H2O + 6O2. For NEET, remember the 12-water version as the correct balanced equation.

What do the reaction conditions above the arrow mean?

The arrow carries 'light' and 'chlorophyll' because photosynthesis needs light energy captured by chlorophyll pigments. Without light or chlorophyll the reaction does not proceed, which is why they are written as conditions, not as reactants or products.

⚠️ The NEET trap
Choosing 6CO2 + 6H2O → C6H12O6 + 6O2 as the correct balanced equation.
The correct balanced equation is 6CO2 + 12H2O → C6H12O6 + 6H2O + 6O2, because 12 water molecules are split to release 6 O2.
🧠 6 water is the empirical (old) form; 12 water is the correct form. NTA loves testing '12 water molecules' and 'O2 comes from water'.

Real NEET questions

NEET 2018

Oxygen is not produced during photosynthesis by

A · Cycas
B · Nostoc
C · Green sulphur bacteria
D · Chara
Solution: The O2 in photosynthesis comes from splitting of water (H2O is the hydrogen donor in green plants, cyanobacteria like Nostoc, algae like Chara, and Cycas). Green sulphur bacteria use H2S, not H2O, as the hydrogen donor, so their oxidation product is sulphur or sulphate, not O2 (anoxygenic photosynthesis). This links directly to the overall equation: 12 H2O are split to release 6 O2 only when water is the donor.

Solved Photosynthesis in Higher Plants NEET PYQs

Try the real previous-year questions from this chapter — each with the answer and a full solution.

See all 32 Photosynthesis in Higher Plants NEET PYQs ›
Next concept: Van Niel's Experiment and the Hydrogen Donor ConceptKeep learning — 2 minFeeling ready? Solve the Photosynthesis in Higher Plants NEET PYQs ›Or practice on your phone — get the free MedicNEET app ›

Frequently asked

What is the correct overall equation of photosynthesis?

6CO2 + 12H2O → C6H12O6 + 6H2O + 6O2, taking place in the presence of light and chlorophyll.

Where does the oxygen in photosynthesis come from?

From water. Water is split (photolysis) and oxidised to O2. The oxygen does not come from carbon dioxide.

Why does the equation use 12 water molecules?

Because 12 water molecules must be split to release 6 molecules of O2, since the oxygen comes only from water.

Who proved that O2 comes from water?

Cornelius van Niel proposed water is the hydrogen donor; it was later confirmed using radioisotope (heavy oxygen) techniques.

What is the difference between the empirical and correct equation?

The empirical equation uses 6 water (6CO2 + 6H2O → C6H12O6 + 6O2). The correct equation uses 12 water and shows 6 water re-formed as product.