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NEET 2016 Phase 1 · BiologyPrevious Year Question

The taq polymerase enzyme is obtained from:

Answer: (A) Thermus aquaticus. Answer: (A) Thermus aquaticus Solution: Repeated PCR amplification requires a thermostable DNA polymerase (Taq polymerase) that stays active through the high-temperature denaturation step; NCERT states it is isolated from the bacterium Thermus aquaticus.

  1. A.Thermus aquaticus✓
  2. B.Thiobacillus ferroxidans
  3. C.Bacillus subtilis
  4. D.Pseudomonas putida

Correct Answer

(A) Thermus aquaticus

Solution & Explanation

Answer: (A) Thermus aquaticus Solution: Repeated PCR amplification requires a thermostable DNA polymerase (Taq polymerase) that stays active through the high-temperature denaturation step; NCERT states it is isolated from the bacterium Thermus aquaticus. Thiobacillus ferroxidans (bioleaching), Bacillus subtilis and Pseudomonas putida (the 'superbug') are unrelated to PCR enzymes. The 'thermostable' property is precisely why a thermophile source is needed. NCERT Reference: Ch 9, p.173, line(s) 10 — "polymerase (isolated from a bacterium, Thermus aquaticus), which"

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