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NEET 2016 Phase 2 · BiologyPrevious Year Question

Stirred-tank bioreactors have been designed for

Answer: (C) Availability of oxygen throughout the process. Answer: (C) Availability of oxygen throughout the process Solution: In a stirred-tank bioreactor the stirrer (agitator) does two jobs: it mixes the contents evenly and it ensures oxygen is available throughout the vessel; air may also be bubbled (sparged) through.

  1. A.Purification of product
  2. B.Addition of preservatives to the product
  3. C.Availability of oxygen throughout the process✓
  4. D.Ensuring anaerobic conditions in the culture vessel

Correct Answer

(C) Availability of oxygen throughout the process

Solution & Explanation

Answer: (C) Availability of oxygen throughout the process Solution: In a stirred-tank bioreactor the stirrer (agitator) does two jobs: it mixes the contents evenly and it ensures oxygen is available throughout the vessel; air may also be bubbled (sparged) through. Aerobic microbial/cell cultures need a continuous oxygen supply, so the stirring design is meant to provide this, not anaerobic conditions. Purification and preservative addition belong to downstream processing, not the bioreactor itself. NCERT Reference: Ch 9, p.174, line(s) 4-5 — "mixing and oxygen availability throughout the bioreactor."

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