NEET 2019 Odisha · BiologyPrevious Year Question
Match the following enzymes with their functions : (a) Restriction endonuclease (i) Joins the DNA fragements (b) Restriction exonuclease (ii) Extends primers on genomic DNA template (c) DNA ligase (iii) Cuts DNA at specific position (d) Taq polymerase (iv) Removes nucleotides from the ends of DNA Select the correct option from the following
Answer: (B) a-iii, b-iv, c-i, d-ii. Answer: (2) a-iii, b-iv, c-i, d-ii Solution: A restriction endonuclease cuts DNA at a specific position (iii).
- A.a-iii, b-i, c-iv d-ii
- B.a-iii, b-iv, c-i, d-ii✓
- C.a-iv, b-iii, c-i, d-ii
- D.a-ii, b-iv, c-i, d-iii
Correct Answer
(B) a-iii, b-iv, c-i, d-ii
Solution & Explanation
Answer: (2) a-iii, b-iv, c-i, d-ii Solution: A restriction endonuclease cuts DNA at a specific position (iii). An exonuclease removes nucleotides from the ends of the DNA (iv). DNA ligase joins the cut DNA fragments end-to-end (i). Taq (DNA) polymerase extends the primers on the genomic DNA template during PCR (ii). This matching is a-iii, b-iv, c-i, d-ii = option 2. NCERT Reference: Ch 9, p.166, line(s) 7-8 — "Exonucleases remove nucleotides from the ends of the DNA whereas, endonucleases make cuts at specific positions within the DNA"
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