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NEET 2019 Odisha · BiologyPrevious Year Question

The two antibiotic resistance genes on vector pBR322 are for

Answer: (A) Ampicillin and Tetracycline. Answer: (1) Ampicillin and Tetracycline Solution: The cloning vector pBR322 carries two antibiotic-resistance selectable-marker genes, ampR (ampicillin) and tetR (tetracycline); foreign DNA ligated at the BamHI site inactivates tetracycline resistance, allowing recombinant selection.

  1. A.Ampicillin and Tetracycline✓
  2. B.Ampicillin and Chloramphenicol
  3. C.Chloramphenicol and Tetracycline
  4. D.Tetracycline and Kanamycin

Correct Answer

(A) Ampicillin and Tetracycline

Solution & Explanation

Answer: (1) Ampicillin and Tetracycline Solution: The cloning vector pBR322 carries two antibiotic-resistance selectable-marker genes, ampR (ampicillin) and tetR (tetracycline); foreign DNA ligated at the BamHI site inactivates tetracycline resistance, allowing recombinant selection. Chloramphenicol and kanamycin are listed by NCERT only as general examples of selectable markers, not as the genes on pBR322. The Figure 9.4 caption explicitly names ampR and tetR. NCERT Reference: Ch 9, p.169, line(s) 60 — "(ampR and tetR). rop codes for"

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