Match the organism with its use in biotechnology (a) Bacillus (b) Thermus aquaticus (c) Agrobacterium tumefaciens (d) Salmonella typhimurium (i) Cloning vector (ii) Construction of first rDNA (iii) DNA polymerase (iv) Cry proteins Select the correct option from the following:
Answer: (D) (a) - (iv); (b) - (iii); (c) - (i); (d) - (ii). Answer: (D) (a) - (iv); (b) - (iii); (c) - (i); (d) - (ii) Solution: (answer_source external; re-derived as NCERT-correct) Bacillus thuringiensis is the source of Cry (insecticidal) proteins; Thermus aquaticus yields the thermostable DNA polymerase used in PCR; Agrobacterium tumefaciens provides the Ti-plasmid-derived cloning vector for plants; and the first recombinant DNA was constructed using a native plasmid of Salmonella typhimurium by Cohen and Boyer.
- A.(a) - (iii); (b) - (ii); (c) - (iv); (d) - (i)
- B.(a) - (iii); (b) - (iv); (c) - (i); (d) - (ii)
- C.(a) - (ii); (b) - (iv); (c) - (iii); (d) - (i)
- D.(a) - (iv); (b) - (iii); (c) - (i); (d) - (ii)✓
Correct Answer
(D) (a) - (iv); (b) - (iii); (c) - (i); (d) - (ii)
Solution & Explanation
Answer: (D) (a) - (iv); (b) - (iii); (c) - (i); (d) - (ii) Solution: (answer_source external; re-derived as NCERT-correct) Bacillus thuringiensis is the source of Cry (insecticidal) proteins; Thermus aquaticus yields the thermostable DNA polymerase used in PCR; Agrobacterium tumefaciens provides the Ti-plasmid-derived cloning vector for plants; and the first recombinant DNA was constructed using a native plasmid of Salmonella typhimurium by Cohen and Boyer. This matching gives (a)-iv, (b)-iii, (c)-i, (d)-ii = option D. (Cry proteins/Bacillus are detailed in Ch 10; here the Salmonella, Thermus and Agrobacterium facts are chapter-grounded as cited.) NCERT Reference: Ch 9, p.164, line(s) 42-44 — "native plasmid (autonomously replicating circular extra-chromosomal DNA) of Salmonella typhimurium. Stanley Cohen and Herbert Boyer accomplished this in 1972"