Which of the following is not an application of PCR (Polymerase Chain Reaction)
Answer: (A) Purification of isolated protein. Answer: (A) Purification of isolated protein Solution: PCR amplifies DNA in vitro, so its applications are DNA/gene-based — gene amplification, detection of gene mutations, and molecular diagnosis.
- A.Purification of isolated protein✓
- B.Detection of gene mutation
- C.Molecular diagnosis
- D.Gene amplification
Correct Answer
(A) Purification of isolated protein
Solution & Explanation
Answer: (A) Purification of isolated protein Solution: PCR amplifies DNA in vitro, so its applications are DNA/gene-based — gene amplification, detection of gene mutations, and molecular diagnosis. Purifying an already-isolated protein involves separation/downstream techniques, not nucleic-acid amplification, so it is NOT a PCR application. NCERT defines PCR as synthesising multiple copies of the gene/DNA of interest in vitro. NCERT Reference: Ch 9, p.172, line(s) 16-17 — "In this reaction, multiple"