A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
Answer: (A) Palindromic Nucleotide sequences. Answer: (A) Palindromic Nucleotide sequences Solution: Each restriction endonuclease inspects the DNA and, on finding its specific recognition site, binds and cuts both strands; this recognition site is a specific palindromic nucleotide sequence that reads the same on the two strands when orientation of reading is kept the same.
- A.Palindromic Nucleotide sequences✓
- B.Poly(A) tail sequences
- C.Degenerate primer sequence
- D.Okazaki sequences
Correct Answer
(A) Palindromic Nucleotide sequences
Solution & Explanation
Answer: (A) Palindromic Nucleotide sequences Solution: Each restriction endonuclease inspects the DNA and, on finding its specific recognition site, binds and cuts both strands; this recognition site is a specific palindromic nucleotide sequence that reads the same on the two strands when orientation of reading is kept the same. Poly(A) tails, degenerate primers and Okazaki fragments are unrelated to restriction-enzyme recognition. NCERT Reference: Ch 9, p.166, line(s) 13-14 — "Each restriction endonuclease recognises a specific palindromic nucleotide sequences in the DNA."