Statements related to human insulin are given below: Which statement(s) is/are correct about genetically engineered Insulin? (a) Pro-hormone insulin contain extra stretch of C-peptide (b) A peptide and B-peptide chains of insulin were produced separately in E. coli, extracted and combined by creating a disulphide bond between them. (c) Insulin used for treating Diabetes was extracted from Cattles and Pigs. (d) Pro-hormone insulin needs to be processed for converting into a mature and functional hormone. (e) Some patients develop allergic reactions to the foreign insulin. Choose the most appropriate answer from the options given below: (NEET 2022)

Solution & Explanation

Answer: (B) (b) only Solution: For genetically engineered insulin, chains A and B were produced separately in E. coli, extracted and combined by creating disulphide bonds — statement (b). The official key treats only (b) as specifically describing the engineered product, since (a)/(d) describe the natural pro-hormone, (c) describes old animal-source insulin, and (e) refers to allergy to foreign (animal) insulin. Note: statements (a) and (d) are also factually true of insulin biology, making this a commonly contested item. NCERT Reference: Ch 10, p.182, line 13 — "Chains A and B were produced separately, extracted and combined by creating disulfide bonds to form human insulin"