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NEET 2025 · BiologyPrevious Year Question

Which of the following enzyme(s) are NOT essential for gene cloning? A. Restriction enzymes B. DNA ligase C. DNA mutase D. DNA recombinase E. DNA polymerase Choose the correct answer from the options given below :

Answer: (C) C and D only. Answer: (C) C and D only Solution: The key tools of recombinant DNA technology are restriction enzymes, polymerase enzymes, ligases, vectors and the host organism; DNA polymerase is used for amplification (PCR) and is part of the toolkit.

  1. A.D and E only
  2. B.B and C only
  3. C.C and D only✓
  4. D.A and B only

Correct Answer

(C) C and D only

Solution & Explanation

Answer: (C) C and D only Solution: The key tools of recombinant DNA technology are restriction enzymes, polymerase enzymes, ligases, vectors and the host organism; DNA polymerase is used for amplification (PCR) and is part of the toolkit. 'DNA mutase' (C) and 'DNA recombinase' (D) are not enzymes listed as essential for gene cloning, so C and D are NOT essential. Restriction enzymes, DNA ligase and DNA polymerase are all genuinely required. NCERT Reference: Ch 9, p.165, line(s) 28-31 — "recombinant DNA technology can be accomplished only if we have the key tools, i.e., restriction enzymes, polymerase enzymes, ligases, vectors and the host organism."

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