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NEET 2026 (Phase 1) · BiologyPrevious Year Question

Insertion of a foreign DNA at BamHI site in an E.coli cloning vector pBR322 results in the loss of antibiotic resistance towards:

Answer: (C) Tetracycline. Answer: (3) Tetracycline Solution: In pBR322 the BamHI site lies within the tetracycline resistance gene.

  1. A.Ampicillin and tetracycline
  2. B.Ampicillin
  3. C.Tetracycline✓
  4. D.Gentamycin

Correct Answer

(C) Tetracycline

Solution & Explanation

Answer: (3) Tetracycline Solution: In pBR322 the BamHI site lies within the tetracycline resistance gene. Insertion of foreign DNA at this site disrupts (inactivates) the tetracycline resistance gene, so the recombinant plasmids lose tetracycline resistance while still retaining ampicillin resistance (used for selection). Gentamycin is not a marker on pBR322. NCERT Reference: Ch 9, p.169, line(s) 39-43 — "you can ligate a foreign DNA at the BamH I site of tetracycline resistance gene in the vector pBR322. The recombinant plasmids will lose tetracycline resistance due to insertion of foreign DNA"

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