Class 12 · Biotechnology and Its Applications

Protoplast Fusion and Somatic Hybrids — NEET Biology

✅ Asked in NEET 2025
✅ NEET 2025 PYQ · Asked 2 times

Which of the following are fused in somatic hybridization involving two varieties of plants?

Q1 of 2NEET 2025

Which of the following are fused in somatic hybridization involving two varieties of plants?

Q2 of 2NEET 2024

Which of the following are fused in somatic hybridization involving two varieties of plants? (NEET 2024)

Answer & NCERT explanation

Correct answer: D Protoplasts

In somatic hybridization, protoplasts (cells with cell walls removed) from two different plant varieties are fused together. This technique allows combining genetic material from different species. Protoplasts are essential because cell walls would prevent fusion. Callus is undifferentiated tissue, somatic embryos are developmental stages, and pollens are male gametes used in sexual reproduction.

Read more NCERT concept on the PYQ

📖 NCERT Source

Scientists have even isolated single cells from plants and after digesting their cell walls have been able to isolate naked protoplasts (surrounded by plasma membranes). Isolated protoplasts from two different varieties of plants – each having a desirable character – can be fused to get hybrid protoplasts, which can be further grown to form a new plant. These hybrids are called somatic hybrids while the process

NCERT Biology · Class 12 · Chapter 10 · Paragraph 16
How NTA Uses This Concept

NTA tests your understanding of protoplast technology: isolating naked protoplasts (without cell walls) by enzymatic digestion, then fusing protoplasts from two different plant varieties to create hybrid protoplasts with combined desirable traits. The key trap is confusing protoplasts with protoplasm or thinking cell walls can be present during fusion—they cannot. Students often misunderstand that somatic hybridization creates new plant varieties combining traits from both parents without sexual reproduction. Remember: protoplast = plasma membrane + cytoplasm + nucleus (no cell wall), and fusion requires naked protoplasts to make contact for successful merging and hybrid plant development.

❓ Frequently Asked Questions
What does NCERT say about Scientists have even isolated?
Scientists have even isolated single cells from plants and after digesting their cell walls have been able to isolate naked protoplasts (surrounded by plasma membranes). Isolated protoplasts from two different varieties of plants – each having a desirable character – can be fused to get hybrid protoplasts, which can be further grown to form a new plant.
Has this concept appeared in NEET?
Yes — appeared in NEET 2025, 2024. Describes fusion of protoplasts from different plant varieties
Which chapter is this from?
Biotechnology and Its Applications, Class 12 NCERT Biology.

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