ELISA: Antigen-Antibody Detection — NEET Biology
📚 Practice ConceptELISA is based on the principle of antigen-antibody interaction. Infection by pathogen can be detected by the presence of antigens (proteins, glycoproteins, etc.) or by detecting the antibodies synthesised against the pathogen.
Which of the following statements regarding molecular diagnosis techniques are correct? S1. ELISA detects the presence of pathogens by identifying their DNA. S2. PCR can detect very low concentrations of bacteria or viruses before disease symptoms are visible. S3. Autoradiography involves hybridisation of a radioactive probe to its complementary DNA in a clone of cells. S4. ELISA relies on the principle of antigen-antibody interaction to detect infection. S5. A clone having a mutated gene will not appear on the photographic film when detected by autoradiography using a complementary probe.
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