Sticky Ends & DNA Ligation — NEET Biology
📚 Practice Concept📖 NCERT Source
When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of 'sticky-ends' and, these can be joined together (end-to-end) using DNA ligases.
🖼️Related NCERT figure: A flow diagram showing the recombinant DNA technology process. It shows foreign DNA and vector DNA (plasmid) being cut by the same restriction enzyme at specific points, creating sticky ends. The DNA ligase then joins the foreign DNA to the plasmid to create a recombinant DNA molecule. This is then transformed into E.coli (cloning host) where the cells divide to produce multiple copies. (Figure 9.2 Diagrammatic representation of recombinant DNA technology)
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QuestionPractice Question
In the given figure, which step confirms that both foreign DNA and vector plasmid were digested using the same restriction enzyme?
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