Sticky Ends & DNA Ligation — NEET Biology
📚 Practice Concept📖 NCERT Source
When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of 'sticky-ends' and, these can be joined together (end-to-end) using DNA ligases.
📐See NCERT Figure 9.2 for the diagram.
Practice This Concept
QuestionPractice Question
In the given figure, which step confirms that both foreign DNA and vector plasmid were digested using the same restriction enzyme?
📖 Solution & NCERT Explanation
View solution & NCERT explanation
Correct answer: B — Foreign DNA and vector showing compatible sticky ends
The figure shows both foreign DNA and vector DNA being cut at similar positions, forming complementary sticky ends—only possible if the same restriction enzyme was used.
❓ Frequently Asked Questions
What does NCERT say about When cut by same?
When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of 'sticky-ends' and, these can be joined together (end-to-end) using DNA ligases.
Has this concept appeared in NEET?
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Which chapter is this from?
Biotechnology: Principles and Processes, Class 12 NCERT Biology.
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