Compatible Restriction Sites in Recombinant DNA — NEET Biology
🔥 Predicted for NEET 2027📖 NCERT Source
You may have realised that normally, unless one cuts the vector and the source DNA with the same restriction enzyme, the recombinant vector molecule cannot be created.
Why This May Be Tested in NEET 2027
Adjacent to a frequently-asked paragraph in the same chapter.
Practice This Concept
QuestionPredicted for NEET 2027
Q2. Why must both the plasmid vector and source DNA be cut with the same restriction enzyme?
📖 Solution & NCERT Explanation
View solution & NCERT explanation
Correct answer: C — To allow complementary sticky ends for ligation
Sticky ends produced by the same enzyme pair up, enabling successful ligation.
❓ Frequently Asked Questions
What does NCERT say about You may have realised?
You may have realised that normally, unless one cuts the vector and the source DNA with the same restriction enzyme, the recombinant vector molecule cannot be created.
Has this concept appeared in NEET?
High probability for NEET 2027. Adjacent to a frequently-asked paragraph in the same chapter.
Which chapter is this from?
Biotechnology: Principles and Processes, Class 12 NCERT Biology.
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88/90of the NEET 2026 Biology paper matched from the MedicNEET question bank
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