Class 12 · Biotechnology: Principles and Processes

Compatible Restriction Sites in Recombinant DNA — NEET Biology

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You may have realised that normally, unless one cuts the vector and the source DNA with the same restriction enzyme, the recombinant vector molecule cannot be created.

NCERT Biology · Class 12 · Chapter 9 · Paragraph 28
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Adjacent to a frequently-asked paragraph in the same chapter.

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Q2. Why must both the plasmid vector and source DNA be cut with the same restriction enzyme?

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