Class 12 · Biotechnology: Principles and Processes

Gene Inactivation and Recombinant Selection — NEET Biology

🔥 Predicted for NEET 2027
📖 NCERT Source

Gene gets 'inactivated due to insertion' of alien DNA, and helps in selection of recombinants.

NCERT Biology · Class 12 · Chapter 9 · Paragraph 36
Why This May Be Tested in NEET 2027

Adjacent to a frequently-asked paragraph in the same chapter.

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QuestionPredicted for NEET 2027

Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactosidase production and the recombinant plasmid is inserted in an E. coli strain: (NEET 2021)

📖 Solution & NCERT Explanation
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Correct answer: C It will not be able to confer ampicillin resistance to the host cell.

Insertion in the ampR gene disrupts its function, so the recombinant plasmid no longer provides resistance to ampicillin.

❓ Frequently Asked Questions
What does NCERT say about Gene gets inactivated due?
Gene gets 'inactivated due to insertion' of alien DNA, and helps in selection of recombinants.
Has this concept appeared in NEET?
High probability for NEET 2027. Adjacent to a frequently-asked paragraph in the same chapter.
Which chapter is this from?
Biotechnology: Principles and Processes, Class 12 NCERT Biology.

Through deep analysis of NEET and NTA, 176 of the 180 ReNEET 2026 (June 21) questions were already in the MedicNEET question bank before the exam.

176/180
of the ReNEET 2026 paper (all subjects) was already in the MedicNEET question bank

MedicNEET's Biology question bank is built from the same NCERT lines NTA picks repeatedly. Not random MCQs — questions crafted exactly like NTA crafts them.

176 of 180 ReNEET 2026 questions traced to MedicNEET17,000+ Biology questionsHindi + English
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