Class 12 · Biotechnology: Principles and Processes

Gene Inactivation and Recombinant Selection — NEET Biology

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Gene gets 'inactivated due to insertion' of alien DNA, and helps in selection of recombinants.

NCERT Biology · Class 12 · Chapter 9 · Paragraph 36
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Adjacent to a frequently-asked paragraph in the same chapter.

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QuestionPredicted for NEET 2027

Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactosidase production and the recombinant plasmid is inserted in an E. coli strain: (NEET 2021)

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