Heat Shock Transformation of Bacteria — NEET Biology
📚 Practice ConceptRecombinant DNA can then be forced into such cells by incubating the cells with recombinant DNA on ice, followed by placing them briefly at 42°C (heat shock), and then putting them back on ice. This enables the bacteria to take up the recombinant DNA.
Arrange the following steps in the correct chronological order for making bacterial cells competent and facilitating the uptake of recombinant DNA. I. Incubation of cells with recombinant DNA on ice. II. Treatment of bacterial cells with a specific concentration of a divalent cation like calcium. III. Briefly placing the cells at 42°C (heat shock). IV. Transferring the cells back to ice. V. Introduction of recombinant DNA into the surrounding medium of the cells.
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