Recombinant DNA Technology Processes — NEET Biology

Correct answer: D — A–iv, B–ii, C–i, D–iii, E–v

Let's match each item from Column I to its correct description in Column II: A. Restriction Endonuclease: These are 'molecular scissors' that cut DNA at specific palindromic nucleotide sequences. So, A-iv is the correct match. B. DNA Ligase: This enzyme joins cut DNA molecules, particularly their sticky ends, by forming phosphodiester bonds, which is crucial for creating recombinant DNA. So, B-ii is the correct match. C. Ti Plasmid: The tumor-inducing (Ti) plasmid from *Agrobacterium tumifaciens* is modified (disarmed) and used as a cloning vector to deliver genes of interest into plant cells. So, C-i is the correct match. D. Polymerase Chain Reaction (PCR): PCR is a technique that amplifies (makes multiple copies of) a gene or DNA of interest in vitro using two sets of primers and DNA polymerase. So, D-iii is the correct match. E. Micro-injection: This is a method where recombinant DNA is directly injected into the nucleus of an animal cell. So, E-v is the correct match. Therefore, the correct match is A–iv, B–ii, C–i, D–iii, E–v. The core concept tested is the identification and understanding of the specific roles of various tools and techniques employed in recombinant DNA technology, as detailed in NCERT Chapter 9.