Class 12 · Molecular Basis of Inheritance

Hershey-Chase Experiment & DNA Proof — NEET Biology

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How did Hershey and Chase differentiate between DNA and protein in their experiment while proving that DNA is the genetic material?

NCERT Biology · Class 12 · Chapter 5 · Paragraph 168
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Which of the following statements are correct regarding the Hershey and Chase experiment to prove DNA as the genetic material? S1: Viruses were grown in a medium containing radioactive sulfur (35S) to label viral DNA. S2: Viruses grown in the presence of radioactive phosphorus (32P) contained radioactive DNA but not radioactive protein. S3: After infection and blending, bacteria infected with 35S labeled viruses showed radioactivity in the supernatant, not in the bacterial cells. S4: The experiment demonstrated that protein from the viruses entered the bacterial cells during infection. S5: Centrifugation was used to separate the viral particles from the bacterial cells after infection.

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