Theoretically, how many such beads (nucleosomes) do you imagine are present in a mammalian cell?
Adjacent to a frequently-asked paragraph in the same chapter.
Arrange the following events in the correct sequence of DNA helix packaging inside a eukaryotic cell: 1. Formation of chromatin fibres from beads-on-string structure 2. DNA wraps around histone octamer to form nucleosome (200 bp each) 3. Condensation of chromatin fibres into chromosomes at metaphase 4. Organisation of nucleosomes into beads-on-string structure under EM 5. Association of DNA with positively charged histones
Correct answer: A — 5 → 2 → 4 → 1 → 3
DNA (negatively charged) first associates with histones (positively charged). This wrapping around histone octamer creates nucleosomes (200 bp DNA per nucleosome). Nucleosomes appear as beads-on-string under EM. These further coil into chromatin fibres. During metaphase, chromatin fibres are highly condensed to form visible chromosomes.
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