Class 12 · Molecular Basis of Inheritance

Hershey-Chase Experiment & DNA Entry — NEET Biology

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The bacteriophage attaches to the bacteria and its genetic material then enters the bacterial cell. The bacterial cell treats the viral genetic material as if it was its own and subsequently manufactures more virus particles. Hershey and Chase worked to discover whether it was protein or DNA from the viruses that entered the bacteria.

NCERT Biology · Class 12 · Chapter 5 · Paragraph 35
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Which of the following statements are correct regarding the Hershey and Chase experiment to prove DNA as the genetic material? S1: Viruses were grown in a medium containing radioactive sulfur (35S) to label viral DNA. S2: Viruses grown in the presence of radioactive phosphorus (32P) contained radioactive DNA but not radioactive protein. S3: After infection and blending, bacteria infected with 35S labeled viruses showed radioactivity in the supernatant, not in the bacterial cells. S4: The experiment demonstrated that protein from the viruses entered the bacterial cells during infection. S5: Centrifugation was used to separate the viral particles from the bacterial cells after infection.

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