Class 12 · Molecular Basis of Inheritance

DNA as Genetic Material — NEET Biology

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From the foregoing discussion, it is clear that the debate between proteins versus DNA as the genetic material was unequivocally resolved from Hershey-Chase experiment. It became an established fact that it is DNA that acts as genetic material. However, it subsequently became clear that

NCERT Biology · Class 12 · Chapter 5 · Paragraph 39
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Arrange the following key steps of Hershey and Chase’s experiment in the correct chronological sequence: 1. Radioactive phages were allowed to infect E. coli bacteria. 2. Some viruses were grown in medium containing radioactive sulfur (³⁵S) → labeled proteins. 3. Some viruses were grown in medium containing radioactive phosphorus (³²P) → labeled DNA. 4. After infection, phage coats were removed by blending and separated by centrifugation. 5. Bacteria infected with ³²P-labeled phages became radioactive, showing DNA entered cells.

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Correct answer: B 3 → 2 → 1 → 4 → 5

First, bacteriophages were labeled in two ways: with ³²P for DNA and with ³⁵S for protein. Then both types infected E. coli. Blending and centrifugation separated viral coats from bacteria. Radioactivity was detected only inside bacteria infected with ³²P-labeled DNA, proving DNA is the genetic material.

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From the foregoing discussion, it is clear that the debate between proteins versus DNA as the genetic material was unequivocally resolved from Hershey-Chase experiment. It became an established fact that it is DNA that acts as genetic material.
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Molecular Basis of Inheritance, Class 12 NCERT Biology.

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