DNA Replication & Base Pairing — NEET Biology
📚 Practice Concept"It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material" (Watson and Crick, 1953).
Which of the following statements correctly support the semi-conservative mode of DNA replication as established by experimental evidence? S1: In Meselson and Stahl’s experiment, the appearance of DNA with intermediate density after one generation in ¹⁴N medium was key evidence. S2: The experiment by Taylor and colleagues using radioactive thymidine in Vicia faba chromosomes further confirmed semi-conservative replication in eukaryotes. S3: If DNA replication were conservative, a single band of hybrid density would be observed after two generations in a light medium. S4: The discontinuous synthesis of one of the DNA strands necessitates the action of DNA ligase, which is characteristic of semi-conservative replication. S5: The phenomenon of complementary base pairing suggests the possibility of a copying mechanism for genetic material, supporting the semi-conservative model.
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