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NEET 2020 · BiologyPrevious Year Question

In gel electrophoresis, separated DNA fragments can be visualized with the help of

Answer: (D) Ethidium bromide in UV radiation. Answer: (D) Ethidium bromide in UV radiation Solution: (answer_source external; re-derived from NCERT.) Separated DNA fragments are not visible in ordinary light, so they are stained with ethidium bromide and then exposed to UV radiation, producing bright orange-coloured bands.

  1. A.Acetocarmine in UV radiation
  2. B.Ethidium bromide in infrared radiation
  3. C.Acetocarmine in bright blue light
  4. D.Ethidium bromide in UV radiation✓

Correct Answer

(D) Ethidium bromide in UV radiation

Solution & Explanation

Answer: (D) Ethidium bromide in UV radiation Solution: (answer_source external; re-derived from NCERT.) Separated DNA fragments are not visible in ordinary light, so they are stained with ethidium bromide and then exposed to UV radiation, producing bright orange-coloured bands. Acetocarmine and infrared/blue-light combinations are not used for this purpose. NCERT Reference: Ch 9, p.168, line(s) 19-20 — "with a compound known as ethidium"

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