Which of the following is not a cloning vector?
Answer: (D) Probe. Answer: (D) Probe Solution: Cloning vectors are DNA molecules that carry a foreign DNA fragment and replicate it inside a host; BAC and YAC (bacterial/yeast artificial chromosomes) and the plasmid pBR322 are all cloning vectors.
- A.BAC
- B.YAC
- C.pBR322
- D.Probe✓
Correct Answer
(D) Probe
Solution & Explanation
Answer: (D) Probe Solution: Cloning vectors are DNA molecules that carry a foreign DNA fragment and replicate it inside a host; BAC and YAC (bacterial/yeast artificial chromosomes) and the plasmid pBR322 are all cloning vectors. A probe is a labelled single-stranded nucleic acid used to detect a complementary sequence by hybridisation, not to clone DNA, so it is not a cloning vector. NCERT Reference: Ch 9, p.165, line(s) 4 — "then linked with the plasmid DNA. These plasmid DNA act as vectors to"