Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence. A. Insertion of recombinant DNA into the host cell. B. Cutting of DNA at specific location by restriction enzyme. C. Isolation of desired DNA fragment. D. Amplification of gene of interest using PCR. Choose the correct answer from the options given below:
Answer: (C) C, B, D, A. Answer: (C) C, B, D, A Solution: Recombinant DNA technology proceeds in sequence: isolation of the desired DNA fragment (C), cutting of DNA at specific locations by restriction enzyme (B), amplification of the gene of interest using PCR (D), and insertion of the recombinant DNA into the host cell (A).
- A.B, C, D, A
- B.C, A, B, D
- C.C, B, D, A✓
- D.B, D, A, C
Correct Answer
(C) C, B, D, A
Solution & Explanation
Answer: (C) C, B, D, A Solution: Recombinant DNA technology proceeds in sequence: isolation of the desired DNA fragment (C), cutting of DNA at specific locations by restriction enzyme (B), amplification of the gene of interest using PCR (D), and insertion of the recombinant DNA into the host cell (A). NCERT lists these steps in the same order. NCERT Reference: Ch 9, p.171, line(s) 18-23 — "Recombinant DNA technology involves several steps in specific"