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NEET 2023 Phase 2 · BiologyPrevious Year Question

Which of the following statement is incorrect about Agrobacterium tumifaciens?

Answer: (C) It is used to deliver gene of interest in both prokaryotic as well as eukaryotic host cells.. Answer: (C) It is used to deliver gene of interest in both prokaryotic as well as eukaryotic host cells.

  1. A.It transforms normal plant cells into tumorous cells.
  2. B.It delivers ‘T-DNA’ into plant cell.
  3. C.It is used to deliver gene of interest in both prokaryotic as well as eukaryotic host cells.✓
  4. D.‘Ti’ plasmid from Agrobacterium tumifaciens used for gene transfer is not pathogenic to plant cells.

Correct Answer

(C) It is used to deliver gene of interest in both prokaryotic as well as eukaryotic host cells.

Solution & Explanation

Answer: (C) It is used to deliver gene of interest in both prokaryotic as well as eukaryotic host cells. Solution: Agrobacterium tumefaciens (via its Ti plasmid / T-DNA) is a vector for transferring genes into plant (eukaryotic) cells — it transforms normal plant cells into tumours and the disarmed Ti plasmid delivers genes into plants; it is not used to deliver genes into prokaryotic hosts, so statement (C) is incorrect. Statements about transforming normal plant cells to tumorous cells, delivering T-DNA, and the modified Ti plasmid being no longer pathogenic are all true per NCERT. NCERT Reference: Ch 9, p.170, line(s) 26 — "to deliver a piece of DNA known as ‘T-DNA’ to transform normal"

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