Thermostable DNA polymerase used in PCR was isolated from :
Answer: (C) Thermus aquaticus. Answer: (C) Thermus aquaticus Solution: PCR amplification requires repeated cycles of high-temperature denaturation, so the DNA polymerase used must be thermostable.
- A.Agrobacterium tumifaciens
- B.Bacillus thuringiensis
- C.Thermus aquaticus✓
- D.Escherichia coli
Correct Answer
(C) Thermus aquaticus
Solution & Explanation
Answer: (C) Thermus aquaticus Solution: PCR amplification requires repeated cycles of high-temperature denaturation, so the DNA polymerase used must be thermostable. This thermostable DNA polymerase (Taq polymerase) is isolated from the bacterium Thermus aquaticus and remains active during the high-temperature denaturation step. The other listed bacteria are not the source of this enzyme. NCERT Reference: Ch 9, p.173, line(s) 9-10 — "the use of a thermostable DNA polymerase (isolated from a bacterium, Thermus aquaticus), which remain active during the high temperature induced denaturation"