NEET 2026 (Phase 1) · BiologyPrevious Year Question
Arrange the following steps of DNA fingerprinting in a correct sequence. A. Isolation of DNA and its digestion by restriction endonucleases. B. Hybridisation using a labelled VNTR probe. C. Transferring of separated DNA fragments to synthetic membranes. D. Detection of hybridised DNA fragments by autoradiography. E. Separation of DNA fragments by electrophoresis. Choose the correct answer from the options given below :
Answer: (C) A, E, C, B, D. Answer: (C) A, E, C, B, D.
- A.A, B, D, C, E
- B.A, D, B, E, C
- C.A, E, C, B, D✓
- D.A, E, B, C, D
Correct Answer
(C) A, E, C, B, D
Solution & Explanation
Answer: (C) A, E, C, B, D. The correct order is: isolation and digestion of DNA (A), separation of fragments by electrophoresis (E), transfer (blotting) to synthetic membranes (C), hybridisation using labelled VNTR probe (B), and detection by autoradiography (D). NCERT Ch 5, p.106, lines 023-029: "(i) isolation of DNA, (ii) digestion of DNA by restriction endonucleases, (iii) separation of DNA fragments by electrophoresis, (iv) transferring (blotting) of separated DNA fragments to synthetic membranes... (v) hybridisation using labelled VNTR probe, and (vi) detection of hybridised DNA fragments by autoradiography."
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