Restriction Endonucleases: DNA Cutting — NEET Biology
📚 Practice ConceptRestriction endonucleases are used in genetic engineering to form 'recombinant' molecules of DNA, which are composed of DNA from different sources/genomes.
Which of the following statements about restriction enzymes and the DNA fragments they generate are NOT correct? S1: Restriction enzymes always cut DNA molecules at the exact center of palindromic recognition sequences. S2: Sticky ends are single-stranded overhangs that readily form hydrogen bonds with complementary sequences. S3: The formation of recombinant DNA molecules requires cutting both the vector and the alien DNA with different restriction enzymes to ensure specific ligation. S4: The stickiness of DNA ends facilitates the action of DNA ligase by directly promoting stable covalent bond formation between the sugar-phosphate backbones. S5: Exonucleases remove nucleotides from the ends of DNA, while endonucleases make cuts at specific positions within the DNA.
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