They also discovered that protein-digesting enzymes (proteases) and RNA-digesting enzymes (RNases) did not affect transformation, so the transforming substance was not a protein or RNA. Digestion with DNase did inhibit transformation, suggesting that the DNA caused the transformation. They concluded that DNA is the hereditary material, but not all biologists were convinced.
Consider the following statements regarding the biochemical characterisation of the transforming principle by Avery, MacLeod, and McCarty: S1. They concluded that DNA is the hereditary material responsible for transformation. S2. Treatment of the heat-killed S strain extract with protease prevented transformation. S3. Treatment of the heat-killed S strain extract with RNase destroyed the transforming ability. S4. Their work was a continuation of Frederick Griffith’s experiment to identify the nature of the transforming principle. S5. Before their experiment, RNA was widely believed to be the genetic material. Which of the following statements are NOT correct?
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