Assertion A: In recombinant DNA technology, lysozyme is used for disrupting bacterial cells while cellulase is used for plant cells. Reason R: Isolation of genetic material needs disruption of cells. Choose the most appropriate answer:
- A.Both A and R are correct and R is the correct explanation of A✓
- B.Both A and R are correct but R is not the correct explanation of A
- C.A is correct but R is not correct
- D.A is not correct but R is correct
Correct Answer
(A) Both A and R are correct and R is the correct explanation of A
Solution & Explanation
To isolate DNA the cell must be broken open. The cell walls are digested with enzymes specific to the organism: lysozyme for bacteria, cellulase for plant cells, chitinase for fungi (Assertion correct). This is done precisely because genetic material is enclosed within membranes and must be released by cell disruption (Reason correct and the explanation).
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