Sample Questions — Replication
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MCQ
15. What was the key finding from Meselson and Stahl’s experiment after two generations?
✅ Correct Answer: D
This result confirms semiconservative nature of DNA replication.
MCQ
17. The enzyme required to seal gaps between Okazaki fragments is:
✅ Correct Answer: B
DNA ligase joins the sugar-phosphate backbone to seal nicks.
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MCQ
5. What is the role of the template strand during replication?
✅ Correct Answer: C
The parental strand acts as a template to build a new strand.
MCQ
17. Why did the density of DNA decrease in the second generation?
✅ Correct Answer: D
DNA synthesized after transferring to 14N medium becomes lighter.
MCQ
2. Match the following:
Column I (Event)
A. ¹⁵N incorporation
B. Transfer to ¹⁴N mediumC.
One generation after transfer
D. Two generations after transfer
Column II (Description)
I. Intermediate density DNA
II. Equal light and hybrid DNA
III. Heavy DNA formation
IV. Initiates hybrid DNA formation
✅ Correct Answer: C
When E. coli is grown in a medium containing heavy nitrogen (¹⁵N), its DNA becomes denser (“heavy DNA”). When these cells are shifted to a medium containing light nitrogen (¹⁴N), newly synthesized DNA strands incorporate ¹⁴N, producing hybrid (¹⁵N–¹⁴N) DNA after one generation. After two generations, half the DNA is hybrid and half is completely light (¹⁴N–¹⁴N), confirming the semiconservative mode of replication.
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